Assistant Professor Dr.Nutjeera Intasai
NUTJEERA INTASAI, PhD Member of AMS Cancer Research Unit and MT Cancer Research Unit, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand Nutjeera Intasai is a lecturer at Division of Medical Microscopy, Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University (Chiang Mai, Thailand) since 2000. She obtained her PhD at Mahidol University (Bangkok, Thailand) in 2005. She completed her doctoral research on "Discovery of CD147 molecular function by phage-displayed CD147 ectodmain via gpVIII". Her doctoral research was conducted at Department of Medical Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Thailand and Manitoba Institute of Cell Biology, CancerCare Manitoba, Winnipeg, Manitoba, Canada. Currently, her research is focused on cancer therapy and early diagnosis.
1. 2001-2005: Scholarship from the Commission on Higher Education, Ministry of University Affair, Thailand
2. 2003-2004: Scholarship from Canadian Institutes of Health Research (CIHR) Strategic Training Program Innovative Technologies in Multidisciplinary Health Training, Canada
3. 2010: APCCB 2010 Young Scientist Scholarship from the 12th Asian-Pacific Congress of Clinical Biochemistry (APCCB 2010)
4. 2013-2014: 2013 American Cancer Society International Fellowships for Beginning Investigators (ACSBI) Funded by American Cancer Society
B.Sc. (Medical Technology), Chiang Mai University, Chiang Mai, Thailand
M.S. (Medical Technology), Chiang Mai University, Chiang Mai, Thailand
Ph.D. (Medical Technology), Mahidol University, Bangkok, Thailand
Selected current projects
1. Tositarat T, Intasai S, Chanarat P. Platelet function in aging. Bull Chiang Mai AMS 1997; 30(Suppl 1): S70-S74.
2. Chotayaporn M, Intasai N, Chanarat P. Preparation of urine sediment for being learning and teaching materials. Bull Chiang Mai AMS 2001; 34(1) : 31-7.
3. Intasai N, Chotayaporn M, Chanarat P. Effects of soybean extracts on phagocytosis. Bull Chiang Mai AMS 2001; 34(3): 180-9.
4. Intasai N, Arooncharus P, Kasinrerk W, Tayapiwatana C. Construction of high-density display of CD147 ectodomain on VCSM13 phage via gpVIII: effects of temperature, IPTG, and helper phage infection-period. Protein Expr Purif 2003; 32(2):323-31.
5. Intasai N, Mai S, Kasinrerk W, Tayapiwatana C. Binding of multivalent CD147 phage induces apoptosis of U937 cells. International Immunology 2006; 18(7): 1159-69.
6. Tragoolpua K, Intasai N, Kasinrerk W, Mai S, Yuan Y, Tayapiwatana C. Generation of functional scFv intrabody to abate the expression of CD147 surface molecule of 293A cells. BMC Biotechnology 2008 Jan 29: 8; 5.
7. Intasai N, Trakoolpua K, Pingmuang P, Khunkeawla P, Kasinrerk W, Lieber A, Tapiwatana C. Potent inhibition of OKT3-induced T cell proliferation and suppression of CD147 cell surface expression in HeLa cells by scFv-M6-1B9. Immunobiology 2009; 214(6):410-21.
8. Phongpradist R, Chittasupho C, Intasai N, Teruna J Siahaan TJ, Berkland CJ, Anuchapreeda S, Ampasavate C. Biodegradable Nanoparticles Surface Modification Techniques with cIBR Peptide Targeting to LFA-1 Expressing Leukemic Cells. J Nanotech Eng Med 2012;3: 041005-1-041005-9.
9. Panich T, Mangchak P, Pata S, Tragoolpua K, Tayapiwatana C, Intasai N. CD 147 scFv intrabody-mediated CD147 knockdown inhibits proliferation of human cervical cancer HeLa cells. Bull Chiang Mai Assoc Med Sci 2014;47(1):30-36.
10. Sangboonruang S, Thammasit P, Intasai N, Kasinrerk W, Tayapiwatana C, Tragoolpua K. EMMPRIN reduction via scFv-M6-1B9 intrabody affects a3b1-integrin and MCT1 functions and results in suppression of progressive phenotype in the colorectal cancer cell line Caco-2. Cancer Gene Ther. 2014;21(6):246-55.
11. Poomanee W, Chaiyana W, Intasai N, Leelapornpisit P. Biological activities and characterization of the pod extracts from Sompoi (Acacia concina Linn.) grown in northern Thailand. Int J Pharm Pharm Sci 2015;7(5):237-241.
12. Thammasit P, Sangboonruang S, Suwanpairoj S, Khamaikawin W, Intasai N, Kasinrerk W, Tayapiwatana C, Tragoolpua K. Intracellular Acidosis Promotes Mitochondrial Apoptosis Pathway: Role of EMMPRIN Down-regulation via Specific Single-chain Fv Intrabody. J Cancer. 2015;6(3):276-86.
13. Intasai N, Pata S, Tragoolpua K, Tayapiwatana C. Recombinant multivalent EMMPRIN extracellular domain induces U937 human leukemia cell apoptosis by downregulation of monocarboxylate transporter 1 and activation of procaspase-9. Appl Biochem Biotech 2015;176(6):1781-90.